Block Copolymer Giant Unilamellar Vesicles for High-Throughput Screening

Bottom-up synthetic cells offer the potential to study cellular processes with reduced complexity. Giant unilamellar vesicles (GUVs) can mimic in their morphological characteristics because architecture is precisely controllable. We propose a block copolymer-based GUV system that be used for high-throughput screening. Through droplet microfluidic methods, we produce double emulsions then serve as templates GUVs adjustable inner, polymer membrane, and outer composition. Using flow cytometry, are able analyze tens of thousands short amount time, enabling use screening assays.